Emotional well-being, health and life satisfaction are constantly linked to sustainable conditions which allow people to thrive and flourish. While research inquiries have explored several correlates of emotional well-being, health and life satisfaction, it is only in recent years that the impact of occupation on these factors has come under scientific investigation. There is a growing conscience about the reciprocal relationship between well-being at work, well-being in life, overall health and life satisfaction. India has witnessed exponential growth in the IT sector during the past few years, providing employment opportunities to a majority of its population. Although software professionals have come to symbolise the IT industry, call centres have concurrently evolved as a visible and tangible embodiment of the IT revolution. The present study was conducted to examine and compare the levels of emotional well-being, general health and life satisfaction among employees of the call centres and software industry. A total of 300 individuals, with a mean age of 26.24 years, from call centres (n=150) and software firms (n=150) participated in the study. The Affectometer, General Health Questionnaire and Satisfaction with Life Scale were used to measure the variables. Results from independent sample t-test clearly indicated that call centre employees experienced significantly lower levels of emotional well-being, health and life satisfaction when compared to the software professionals.