Retaining employees in the organization for a longer period of time and simultaneously reducing the rate of employees leaving the organization is one of the crucial challenges faced by all sectors globally. This research paper studies the prime factors which influence the attrition of associate level employees with emphasis on gender specific problems and solutions and the various methodologies adopted by the Hotels in the city of Pune for decreasing the same further and retaining its employees. An exhaustive study of the various HR practices adopted by the Hotels helps us analyze the problem of attrition and list out the most effective factors which influence the human thought procedure as per gender. The research is confined to and emphasizes on the effectiveness of retention measures adopted for retaining the Associate Level employees.